Sword Assay for Human VEGF

Sword Assay for Human VEGF

Vascular endothelial growth factor (VEGF) was identified as a cell specific mitogen, and a mediator of both angiogenesis and vasculogenesis.1 It contributes to the development of tumors, and elevated levels of VEGF production are able to be detected in tumor cells at the extreme periphery of the tumor.2

In response to tumor-induced hypoxia there have been high levels of VEGF found in various types of tumors, but this is not the case in normal tissue.3 Elevated levels of VEGF result in bronchial asthma, diabetic retinopathy, rheumatoid arthritis, and tumor growth and, the biological properties of VEGF make it an important therapeutic target.

TThis Sword Assay has been optimized for and requires the R&D Systems Human VEGF DuoSet ELISA (Catalog No. DY293B).

Assay Type:
Sandwich ELISA

Product #:
SB-HVEGF02-05
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sword assay human vegf performance

See the Sword Performance Difference

  • Sensitivity
  • Precision
  • Recovery
  • Quantification

Sensitivity

Figure 1. Sword Assay for Human VEGF

sword elisa assay vegf vs tmb

Precision

Table 1.
Precision of VEGF Quantification in Human Serum with Sword Assay

sword quantification precision vegf human serum

Table 1. VEGF levels were quantified in human serum from healthy donors using the R&D Systems Human VEGF DuoSet ELISA (DY293B) with Sword Assay for Human VERGF. Donor samples were tested in duplicate in three separate runs.

Table 2.
Precision of VEGF Quantification in Human Plasma EDTA with Sword Assay

sword quantification precision vegf human plasma

Table 2. VEGF levels were quantified in human plasma EDTA from healthy donors using the R&D Systems Human VEGF DuoSet ELISA (DY293B) with Sword Assay for Human VEGF. Donor samples were tested in duplicate in three separate runs.

Recovery

Table 3.
Spike Recovery with Sword Assay for Human VEGF in Serum

sword spike recovery vegf human serum

Table 3. Human VEGF Reference Standard was spiked into pooled human serum from healthy donors. Human VEGF levels were quantified using the R&D Systems Human VEGF DuoSet ELISA (DY293B) with Sword Assay for Human VEGF.

Table 4.
Spike Recovery with Sword Assay for Human VEGF in Plasma EDTA

sword spike recovery vegf human plasma

Table 4. Human VEGF Reference Standard was spiked into pooled human plasma EDTA from healthy donors. Human VEGF levels were quantified using the R&D Systems Human VEGF DuoSet ELISA (DY293B) with Sword Assay for Human VEGF.

Quantification

Table 5.
Quantification of VEGF in Healthy Human Serum

sword quantification vegf human serum

Table 5. Human VEGF was quantified in human serum from twelve healthy donors using the R&D Systems Human VEGF DuoSet ELISA (DY293B) with Sword Assay for Human VEGF. Measured human VEGF levels varied from not detectable (ND) to 14.33 pg/ml. Mean and median human VEGF levels were 4.59 pg/ml and 4.40 pg/ml, respectively.

Table 6.
Quantification of VEGF in Healthy Human Plasma

sword quantification il-1b human plasma

Table 6. Human VEGF was quantified in human plasma EDTA from twelve healthy donors using the R&D Systems Human VEGF DuoSet ELISA (DY293B) with Sword Assay for Human VEGF. Measured human VEGF levels varied from 0.19 pg/ml to 10.02 pg/ml. Mean and median human VEGF levels were 2.79 pg/ml and 2.12 pg/ml, respectively.

Citations

  1. Muller YA, et al.: The crystal structure of vascular endothelial growth factor (VEGF) refined to 1.93 A resolution: multiple copy flexibility and receptor binding. Structure 1997, 5 (10):1325–1338
  2. Neufeld, G, et al.: Vascular endothelial growth factor and its receptors. The FASEB Journal 1999, 13:9-22.
  3. Soker, S, et al.: Characterization of Novel Vascular Endothelial Growth Factor (VEGF) Receptors on Tumor Cells that Bind VEGF166 via its Exon 7-encoded Domain. The Journal of Biological Chemistry 1996, 271:5761-5767.

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