Sword Assay for Human IL-33
IL-33 is a nuclear cytokine that is part of the innate immune response mechanism, and is frequently linked to inflammation in diseases such as asthma, Crohn’s disease, and atopic dermatitis. Interaction between IL-33 and the ST2-1RAcP receptor1 activates signaling pathways in several innate immune lymphoid cells, including natural killer, Th2, basophils, and eosinophils as a means of clearing out invading microorganisms.2,3
IL-33 is constitutively expressed in the cell nuclei of normal healthy individuals.4 Damage to the cell causes the release of IL-33 into the extracellular matrix which then signals the beginning of the inflammatory process.5 As a result, IL-33 functions as an alarmin and subsequently has been identified as a strong biomarker candidate for inflammation.
This Sword Assay has been optimized for use with the R&D Systems Quantikine ELISA for Human IL-33 (Catalog No. D3300). This protocol allows the user to use 100 μL sample sizes.
See the Sword Performance Difference
Sensitivity
Figure 1. Sword Assay for Human IL-33
Precision
Table 1.
Precision of IL-33 Quantification in Human Serum with Sword Assay
Table 1. IL-33 levels were quantifies in human serum from healthy donors using the R&D Systems Human IL-33 Quantikine ELISA (D3300) with Sword Assay for Human IL-33. Donor samples were tested in duplicate in three separate runs.
Table 2.
Precision of IL-33 Quantification in Human Plasma EDTA with Sword Assay
Table 2. IL-33 levels were quantified in human plasma EDTA from healthy donors using the R&D Systems Human IL-33 Quantikine ELISA (D3300) with Sword Assay for Human IL-33. Donor samples were tested in duplicate in three separate runs.
Recovery
Table 3.
Spike Recovery with Sword Assay for Human IL-33 in Serum
Table 3. Human IL-33 Reference Standard was spiked into pooled human serum from healthy donors. Human IL-33 levels were quantifies using the R&D Systems Human IL-33 Quantikine ELISA (D3300) with Sword Assay for Human IL-33.
Table 4.
Spike Recovery with Sword Assay for Human IL-33 in Plasma EDTA
Table 4. Human IL-33 Reference Standard was spiked into pooled human plasma EDTA from healthy donors Human IL-33 levels were quantified using the R&D Systems Human Il-33 Quantikine ELISA (D3300) with Sword Assay for Human IL-33.
Quantification
Table 5.
Quantification of IL-33 in Healthy Human Serum
Table 5. Human IL-33 was quantifies in human serum from twelve healthy donors using the R&D Systems Human IL-33 Quantikine ELISA (D3300) with Sword Assay for Human IL-33. Measured human IL-33 levels varied from 0.15 pg.ml to 87.67 pg/ml. Mean and median human IL-33 levels were 8.63 pg/ml and 1.08 pg/ml, respectively.
Table 6.
Quantification of IL-33 in Healthy Human Plasma
Table 6. Human IL-33 was quantified in human plasma EDTA from twelve healthy donors using the R&D Systems Human IL-33 Quantikine ELISA (D3300) with Sword Assay for Human IL-33. Measured human IL-33 levels varied from 0.19 pg/ml to 9.03 pg/ml. Mean and median human IL-33 levels were 2.27 pg/ml and 1.64 pg/ml respectively.
Citations
- Schmitz J, et al.: IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines. Immunity 2005, 23:479-90.
- Cayrol C, Girard JP: IL-33: an alarmin cytokine with crucial roles in innate immunity, inflammation and allergy. Current opinion in immunology 2014, 31:31-7.
- Liew FY, et al.: Disease-associated functions of IL-33: the new kid in the IL-1 family. Nature reviews Immunology 2010, 10:103-10.
- Moussion C, et al.: The IL-1-like cytokine IL-33 is constitutively expressed in the nucleus of endothelial cells and epithelial cells in vivo: a novel ‘alarmin’? PloS one 2008, 3:e3331.
- Hardman CS, et al.: IL-33 citrine reporter mice reveal the temporal and spatial expression of IL-33 during allergic lung inflammation. European journal of immunology 2013, 43:488-98.
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