Sword Assay for Human IL-23
Interleukin-23 (IL-23) is a pro-inflammatory cytokine composed of two subunits, p19 and p401. Its p40 subunit is also shared with IL-12. However, IL-23 and IL-12 activate distinct cellular pathways.2 IL-23 induces the differentiation of Th17 cells from naïve CD4+ helper T cells to produce IL-17A, IL-17F, IL-6, IL-22, TNFα and GM-CSF. IL-23 also induces tissue macrophages to produce IL-1, TNFα and IL-23.
IL-23 is secreted by both macrophages and dendritic cells as a host defense and wound healing response. It has been implicated in several inflammatory and autoimmune diseases, including psoriasis, psoriatic arthritis, Crohn’s Disease and ankylosing spondylitis. Genome-wide association studies (GWAS) have also linked IL-23 to ulcerative colitis, inflammatory bowel disease (IBD) and graft-versus-host disease.2
This Sword Assay has been optimized for use with the R&D Systems Quantikine ELISA for Human IL-23 (Catalog No. D2300B).
See the Sword Performance Difference
Sensitivity
Figure 1. Sword Assay for Human IL-23
Precision
Table 1.
PPrecision of IL-23 Quantification in Human Serum with Sword Assay
Table 1. IL-23 levels were quantified in human serum from healthy donors using the R&D Systems Human Il-23 Quantikine ELISA (D2300B) with Sword Assay for Human IL-23. Donor samples were tested in duplicate in three separate runs.
Table 2.
Precision of IL-23 Quantification in Human Plasma EDTA with Sword Assay
Table 2. IL-23 levels were quantified in human plasma EDTA from healthy donors using the R&D Systems Human IL-23 Quantikine ELISA (D2300B) with Sword Assay for Human IL-23. Donor samples were tested in duplicate in three separate runs.
Recovery
Table 3.
Spike Recovery with Sword Assay for Human IL-23 in Serum
Table 3. Human IL-23 Reference Standard was spiked into pooled human serum from healthy donors. Human IL-23 levels were quantified using the R&D Systems Human IL-23 Quantikine ELISA (D2300B) with Sword Assay for Human IL-23.
Table 4.
Spike Recovery with Sword Assay for Human IL-23 in Plasma EDTA
Table 4. Human IL-23 Reference Standard was spiked into pooled human plasma EDTA from healthy donors. Human IL-23 levels were quantified using the R&D Systems Human Il-23 Quantikine ELISA (D2300b) with Sword Assay for Human IL-23.
Quantification
Table 5.
Quantification of IL-23 in Healthy Human Serum
Table 5. Human IL-23 was quantified in human serum from twelve healthy donors using the R&D Systems Human IL-23. Measured human IL-23 levels varied from not detectable (ND) to 77.93 pg/ml. Mean and median IL-23 levels were 8.70 pg/ml and 1.09 pg/ml, respectively.
Table 6.
Quantification of IL-23 in Healthy Human Plasma
Table 6. Human IL-23 was quantified in human plasma EDTA from twelve healthy donors using the R&D Systems Human IL-23 Quantikine ELISA (D2300B) with Sword Assay for Human IL-23. Measured human Il-23 levels varied from not detectable (ND) to 5.56 pg/ml. Mean and median human IL-23 levels were 1.03 pg.ml and 0.80 pg/ml, respectively.
Citations
- Duvallet E, et al.: Interleukin-23: a key cytokine in inflammatory diseases. Ann Med 2011, 43(7):503-11.
- Teng MWL, et al.: IL-12 and IL-23 cytokines: from discovery to targeted therapies for immune-mediated inflammatory diseases. Nature Medicine 2015, 21:719-729.
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