Sword Assay for Human IL-1β

Sword Assay for Human IL-1β

Interleukin-1 is a pro-inflammatory cytokine that can be expressed in two separate isoforms: IL-1α and IL-1β.1 IL-1β is expressed by dendritic cells and acts through the TNFα signaling pathway to induce expression of several inflammatory cytokines2, 3; it has therefore been identified as both a biomarker for inflammation diagnosis and a target for inflammation therapy.

Current ELISAs lack the required sensitivity to quantify IL-1β in normal healthy donor serum for proper comparison with diseased individuals. Sword has changed that by developing a reagent based on the principle of Raman Resonance that can easily be inserted into a standard IL-1β ELISA with greatly enhanced sensitivity.

This Sword Assay has been optimized for use with the R&D Systems DuoSet ELISA for Human IL-1β (Catalog No. DY201). This protocol allows the user to use 50 µL sample sizes instead of 100 µL as specified in the DuoSet literature.

Assay Type:
Sandwich ELISA

Product #:
SB-HIL1 β02-05
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sword assay human il-1b performance

See the Sword Performance Difference

  • Sensitivity
  • Precision
  • Recovery
  • Quantification

Sensitivity

Figure 1. Sword Assay for Human IL-1β

sword elisa booster il-1b vs tmb

Precision

Table 1.
Precision of IL-1β Quantification in Human Serum with Sword Assay

sword quantification precision il-1b human serum

Table 1. IL-1β levels were quantified in human serum from healthy donors using the R&D Systems Human IL-1β DuoSet ELISA (DY201) with Sword Assay for Human IL-1β. Donor samples were tested in duplicate in three separate runs.

Table 2.
Precision of IL-1β Quantification in Human Plasma EDTA with Sword Assay

sword quantification precision il-1b human plasma

Table 2. IL-1β levels were quantified in human plasma EDTA from healthy donors using the R&D Systems Human IL-1β DuoSet ELISA (DY201) with Sword Assay for Human IL-1β. Donor samples were tested in duplicate in three separate runs.

Recovery

Table 3.
Spike Recovery with Sword Assay for Human IL-1β in Serum

sword spike recovery il-1b human serum

Table 3. Human IL-1β Reference Standard was spiked into pooled human serum from healthy donors. Human IL-1β levels were quantified using the R&D Systems Human IL-1β DuoSet ELISA (DY201) with Sword Assay for Human IL-1β.

Table 4.
Spike Recovery with Sword Assay for Human IL-1β in Plasma EDTA

sword spike recovery il-1b human plasma

Table 4. Human IL-1β Reference Standard was spiked into pooled human plasma EDTA from healthy donors. Human IL-1β levels were quantified using the R&D Systems Human IL-1β DuoSet ELISA (DY201) with Sword Assay for Human IL-1β.

Quantification

Table 5.
Quantification of IL-1β in Healthy Human Serum

sword quantification il-1b human serum

Table 5. Human IL-1β was quantified in human serum from twelve healthy donors using the R&D Systems Human IL-1β DuoSet ELISA (DY201) with Sword Assay for Human IL-1β.

Table 6.
Quantification of IL-1β in Healthy Human Plasma

sword quantification il-1b human plasma

Table 6. Human IL-1β was quantified in human plasma from twelve healthy donors using the R&D Systems Human IL-1β DuoSet ELISA (DY201) with Sword Assay for Human IL-1β.

Citations

  1. Weber A, et al: Interleukin-1beta (IL-1beta) processing pathway. Sci Signal 2010, 3:cm2.
  2. Luft T, et al: IL-1 beta enhances CD40 ligand-mediated cytokine secretion by human dendritic cells (DC): a mechanism for T cell-independent DC activation. Journal of immunology 2002, 168:713-22.
  3. Zhang et al: Interleukin-1beta induces macrophage inflammatory protein-1beta expression in human hepatocytes. Cell Immunol 2003, 226:45-53.

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