Sword Assay for Human IL-1β
Interleukin-1 is a pro-inflammatory cytokine that can be expressed in two separate isoforms: IL-1α and IL-1β.1 IL-1β is expressed by dendritic cells and acts through the TNFα signaling pathway to induce expression of several inflammatory cytokines2, 3; it has therefore been identified as both a biomarker for inflammation diagnosis and a target for inflammation therapy.
Current ELISAs lack the required sensitivity to quantify IL-1β in normal healthy donor serum for proper comparison with diseased individuals. Sword has changed that by developing a reagent based on the principle of Raman Resonance that can easily be inserted into a standard IL-1β ELISA with greatly enhanced sensitivity.
This Sword Assay has been optimized for use with the R&D Systems DuoSet ELISA for Human IL-1β (Catalog No. DY201). This protocol allows the user to use 50 µL sample sizes instead of 100 µL as specified in the DuoSet literature.
See the Sword Performance Difference
Sensitivity
Figure 1. Sword Assay for Human IL-1β
Precision
Table 1.
Precision of IL-1β Quantification in Human Serum with Sword Assay
Table 1. IL-1β levels were quantified in human serum from healthy donors using the R&D Systems Human IL-1β DuoSet ELISA (DY201) with Sword Assay for Human IL-1β. Donor samples were tested in duplicate in three separate runs.
Table 2.
Precision of IL-1β Quantification in Human Plasma EDTA with Sword Assay
Table 2. IL-1β levels were quantified in human plasma EDTA from healthy donors using the R&D Systems Human IL-1β DuoSet ELISA (DY201) with Sword Assay for Human IL-1β. Donor samples were tested in duplicate in three separate runs.
Recovery
Table 3.
Spike Recovery with Sword Assay for Human IL-1β in Serum
Table 3. Human IL-1β Reference Standard was spiked into pooled human serum from healthy donors. Human IL-1β levels were quantified using the R&D Systems Human IL-1β DuoSet ELISA (DY201) with Sword Assay for Human IL-1β.
Table 4.
Spike Recovery with Sword Assay for Human IL-1β in Plasma EDTA
Table 4. Human IL-1β Reference Standard was spiked into pooled human plasma EDTA from healthy donors. Human IL-1β levels were quantified using the R&D Systems Human IL-1β DuoSet ELISA (DY201) with Sword Assay for Human IL-1β.
Quantification
Table 5.
Quantification of IL-1β in Healthy Human Serum
Table 5. Human IL-1β was quantified in human serum from twelve healthy donors using the R&D Systems Human IL-1β DuoSet ELISA (DY201) with Sword Assay for Human IL-1β.
Table 6.
Quantification of IL-1β in Healthy Human Plasma
Table 6. Human IL-1β was quantified in human plasma from twelve healthy donors using the R&D Systems Human IL-1β DuoSet ELISA (DY201) with Sword Assay for Human IL-1β.
Citations
- Weber A, et al: Interleukin-1beta (IL-1beta) processing pathway. Sci Signal 2010, 3:cm2.
- Luft T, et al: IL-1 beta enhances CD40 ligand-mediated cytokine secretion by human dendritic cells (DC): a mechanism for T cell-independent DC activation. Journal of immunology 2002, 168:713-22.
- Zhang et al: Interleukin-1beta induces macrophage inflammatory protein-1beta expression in human hepatocytes. Cell Immunol 2003, 226:45-53.
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