Frequently Asked Questions

Frequently Asked Questions

1. How does Sword ELISA Booster work?

Sword ELISA Booster uses a patented technology involving resonance Raman. First the Sword Substrate interacts with HRP conjugated to the detection antibody or streptavidin. This converts the substrate molecule to a Raman-active state. When the active molecule is hit by a light photon from the plate-reader light source, it resonates. A second light photon is then reflected by the resonating molecule. This reaction repeated multiple times results in a strong signal from a relatively low abundance of HRP, giving the researcher improved sensitivity and precision at the low end.

2. How do I prepare and use the Sword ELISA Booster?

Click here to access the Quick Reference Guide.

3. My assay is proprietary. Is it still possible to take part in the Sword SWIFT program?

Yes. Sword Bio will sign any confidentiality agreements and material transfer agreements necessary to advance the project. We will also share any and all raw data generated.

4. How do marker-specific boosters differ from the Sword ELISA Booster- Universal or Sword ELISA Booster- Universal for Developers?

All Sword ELISA Boosters contain the four base components required to make and use the detection chemistry. The Sword ELISA Booster- Universal only contains the four base components and a protocol designed to insert the Sword ELISA Booster into any existing sandwich or direct ELISA that uses HRP-conjugates for detection. The Sword ELISA Booster- Universal for Developers additionally contains three Sword Blockers and three Sword Diluents that may be used by the customer for optimizing their assay. The marker-specific boosters contain the optimized protocol as well as any required blockers and diluents for that protocol. These boosters are designed for specific commercial ELISA kits (e.g. R&D Systems Quantikine). The cost for both Boosters is the same; however, the contents vary.

5. Can Sword ELISA Booster be used in any ELISA?

Sword ELISA Booster may be used in any ELISA that uses HRP as the detection. The Sword ELISA Booster is not engineered at this time to work with other detection chemistries, such as alkaline phosphatase for example. The degree of improvement observed with Sword ELISA Booster varies from assay to assay, depending on the quality of the antibodies used and the level of optimization performed.

6. How long and how many times can the Sword ELISA Booster signal be read?

Upon completion of the 30 minute developing time specified in the Sword ELISA Booster protocol, the signal for Sword ELISA Booster is stable for up to 90 minutes. There may be some slight increasing drift, but it will be uniform across the plate. The plate may be read as many times on as many instruments as necessary without any photo-bleaching, quenching or washout.

7. Can Sword ELISA Booster be used in other fluorescent assays like real time PCR?

Sword ELISA Booster is currently only available for ELISA use at this time.

8. Is it OK to mix lots of Sword ELISA Booster if I am using more than 5 plates?

We recommend only combining Sword ELISA Boosters with the same lot number.

9. Can I use black plates? What about 384-well plates?

Sword ELISA Booster may be used on clear or opaque black plates. We do not recommend using white opaque plates. Sword ELISA Booster may also be used on 384-well plates provided that the reagent volumes are scaled accordingly.

10. Can Sword ELISA Booster be used in high-throughput automation platforms?

Sword ELISA Booster is sold in the form of five plate Boosters. If needed, we will work with you to customize boosters for large-scale applications. Sword ELISA Booster is sensitive to air and light, so it will be important to take that into account when scaling up the product.

11. Does Sword ELISA Booster require a dedicated Raman reading instrument?

No. The resonance Raman signal generated when the Sword ELISA Booster interacts with HRP is very similiar to fluorescence and does not require a Raman-specific plate reader. However, successful reading of the Sword ELISA Booster requires a high-intensity light source with an optimal light path that several commercial fluorescent readers are unable to handle. Additionally, it is very important with some exceptions that the instrument be filter-based. The Tecan M1000 from the Infinite series is the only known monochromator-based instrument that works with the Sword ELISA Booster at this time. Other filter-based instruments that are known to work include the BioTek Cytation, BioTek NEO2, Tecan F200, and Molecular Devices Paradigm. A complete list along with applicable settings may be found here.

12. What if I don’t have an instrument that is compatible?

Sword does not want the lack of a suitable instrument to keep you from being successful with your experiments. As a result, we offer to loan an instrument to your facility that will be able to read the Sword ELISA Booster signal. Conditions of this program include an obligation to purchase a pre-negotiated number of Sword ELISA Boosters per month. The instrument may stay at your facility as long as you need, provided you are fulfilling the required orders. We will retrieve the instrument when you are able to purchase an instrument.

For more information,
contact Sword Bio:

General Information

1-800-403-7075 Ext. 101

Technical Support
1-800-403-7075 Ext. 106