Move
Ahead of the Curve in Biomarker Detection
Sword Diagnostics’ ultrasensitive
immunoassay detection platform is now qualified for use
on Tecan’s Infinite® M1000 and 200 Pro Multimode
Microplate Readers.
The new SwordTM Peroxidase Assay provides improved performance
with enhanced signal intensity, providing enhanced sensitivity
and precision in biomarker measurement. This capability
allows you to measure markers not currently used due to
their low abundance, or those that are difficult to detect
using existing technologies. This new technology also provides
the ability to more accurately measure established low abundance
markers. Researchers will appreciate that Sword Peroxidase
Assay easily substitutes into most existing protocols that
use horseradish peroxidase (HRP) with minimal optimization.
Sword’s patented technology provides a significant
and reproducible positive shift in dose-response curves,
providing numerous benefits to assay developers:
• Increased Signal/Background
at low analyte concentrations
• Enhanced sensitivity
– Improved analytical Limit-of-Detection
(LOD)
– Improved marker detectability
• Dose-response curves
shift toward lower analyte concentrations
– Improved Sample Distinction
• Easy integration
into existing and new assays
• Improved precision
and accuracy on low end of the curve
• Reduced sample
sizes
Sword’s technology utilizes a unique substrate that
generates a light scattering, energy exchanged (Raman) signal
that can be quantified using Tecan Infinite M1000 and 200
Pro Multimode Microplate Readers. The principle of the Sword
technology is similar to that of colorimetric assays where
a nearly colorless compound is oxidized by HRP in an ELISA
to form a colored reagent that is quantified by a plate
reader. Sword’s substrate has a very low Raman signal,
but after oxidation by HRP, it has a strong and quantitative
Raman signal that can be measured using the fluorescence
channel of Tecan plate readers.
Reagent Kits
The Raman substrate in the Sword Peroxidase Assay is oxidized
in a highly quantitative manner using HRP conjugates that
are used in most ELISAs without significant assay reformatting.
This allows the Sword reagents to be easily integrated into
existing ELISAs that utilize HRP. Sword Peroxidase Assay
Kits include substrates, buffer, and development solution
concentrate. Custom reagent formulations as well as Sword’s
custom assay development service are available upon request.
Contact us for more information.
Instrumentation Requirements
The Sword chemistry is currently validated on the Tecan
Infinite M1000 and 200 Pro Multimode Microplate Readers. |
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